Comparative analysis of photocaged RGDS peptides for cell patterning
Identifieur interne : 000B40 ( Main/Exploration ); précédent : 000B39; suivant : 000B41Comparative analysis of photocaged RGDS peptides for cell patterning
Auteurs : Catherine A. Goubko [Canada] ; Ajoy Basak [Canada] ; Swapan Majumdar [Canada] ; Harold Jarrell [Canada] ; Nam Huan Khieu [Canada] ; Xudong Cao [Canada]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2013-03.
English descriptors
- Teeft :
- Absorbance, Adhesion, Amide, Amino, Article figure, Avb3, Avb3 integrin, Avb3 integrin receptor, Backbone, Binding energy, Biological applications, Biological systems, Biomaterials, Biomedical materials research, Caging, Caging group, Carboxyl, Carboxyl group, Cell adhesion, Cell patterning, Chem, Cold ether, Control cell adhesion, Crystal structure, Cyclo, Docking, Docking studies, Elisa, Exposure time, Fmoc, Free energy, Goubko, Hydrogel, Integrin, Integrin binding, Integrin receptor, Ligand, Light exposure, Native rgds peptides, Noncaged, Ottawa, Patterning, Peptide, Peptide backbone, Peptide synthesis, Photocaged, Photocaged rgds peptides, Receptor, Rgds, Rgds peptide, Rgds peptides, Side chain, Side chain carboxyl, Uncaged, Uncaging.
Abstract
Photocaged RGDS is a cell nonadhesive tetrapeptide that can be activated with light to become cell‐adhesive. Such molecules can find useful applications in controlling cell adhesion for biological study, drug development, and in forming dynamic, adhesion‐controlled biomaterials. Herein, we prepared RGDS peptide photocaged either on the Arg‐Gly backbone amide nitrogen atom (R[−]GDS) or Asp side chain carboxyl (RG[D]S). A critical comparison of the peptides' chemical and physiological properties relevant for biological applications was carried out. It was observed that RG[D]S was synthesized more readily via automated solid‐phase synthesis, underwent uncaging with a rate constant 3‐fold higher than R[−]GDS, and was more stable in aqueous solution. Automated docking studies were performed to examine the interactions of various caged RGDS peptides with cell surface integrin receptor to identify suitable locations for the photosensitive 2‐nitrobenzyl (NB) group for biological applications. A competitive binding ELISA method compared the ability of various peptides to bind to αVβ3 cell integrin receptors and the data were found to be consistent with the modeling predictions. Finally, the application of our caged RGDS peptides in controlling cell adhesion to form cell patterns on a hydrogel material was presented. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 787–796, 2013.
Url:
DOI: 10.1002/jbm.a.34381
Affiliations:
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6N5</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part A</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title><idno type="ISSN">1549-3296</idno><idno type="eISSN">1552-4965</idno><imprint><biblScope unit="vol">101A</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="787">787</biblScope><biblScope unit="page" to="796">796</biblScope><biblScope unit="page-count">10</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2013-03">2013-03</date></imprint><idno type="ISSN">1549-3296</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1549-3296</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorbance</term><term>Adhesion</term><term>Amide</term><term>Amino</term><term>Article figure</term><term>Avb3</term><term>Avb3 integrin</term><term>Avb3 integrin receptor</term><term>Backbone</term><term>Binding energy</term><term>Biological applications</term><term>Biological systems</term><term>Biomaterials</term><term>Biomedical materials research</term><term>Caging</term><term>Caging group</term><term>Carboxyl</term><term>Carboxyl group</term><term>Cell adhesion</term><term>Cell patterning</term><term>Chem</term><term>Cold ether</term><term>Control cell adhesion</term><term>Crystal structure</term><term>Cyclo</term><term>Docking</term><term>Docking studies</term><term>Elisa</term><term>Exposure time</term><term>Fmoc</term><term>Free energy</term><term>Goubko</term><term>Hydrogel</term><term>Integrin</term><term>Integrin binding</term><term>Integrin receptor</term><term>Ligand</term><term>Light exposure</term><term>Native rgds peptides</term><term>Noncaged</term><term>Ottawa</term><term>Patterning</term><term>Peptide</term><term>Peptide backbone</term><term>Peptide synthesis</term><term>Photocaged</term><term>Photocaged rgds peptides</term><term>Receptor</term><term>Rgds</term><term>Rgds peptide</term><term>Rgds peptides</term><term>Side chain</term><term>Side chain carboxyl</term><term>Uncaged</term><term>Uncaging</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Photocaged RGDS is a cell nonadhesive tetrapeptide that can be activated with light to become cell‐adhesive. Such molecules can find useful applications in controlling cell adhesion for biological study, drug development, and in forming dynamic, adhesion‐controlled biomaterials. Herein, we prepared RGDS peptide photocaged either on the Arg‐Gly backbone amide nitrogen atom (R[−]GDS) or Asp side chain carboxyl (RG[D]S). A critical comparison of the peptides' chemical and physiological properties relevant for biological applications was carried out. It was observed that RG[D]S was synthesized more readily via automated solid‐phase synthesis, underwent uncaging with a rate constant 3‐fold higher than R[−]GDS, and was more stable in aqueous solution. Automated docking studies were performed to examine the interactions of various caged RGDS peptides with cell surface integrin receptor to identify suitable locations for the photosensitive 2‐nitrobenzyl (NB) group for biological applications. A competitive binding ELISA method compared the ability of various peptides to bind to αVβ3 cell integrin receptors and the data were found to be consistent with the modeling predictions. Finally, the application of our caged RGDS peptides in controlling cell adhesion to form cell patterns on a hydrogel material was presented. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 787–796, 2013.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Goubko, Catherine A" sort="Goubko, Catherine A" uniqKey="Goubko C" first="Catherine A." last="Goubko">Catherine A. Goubko</name></noRegion><name sortKey="Basak, Ajoy" sort="Basak, Ajoy" uniqKey="Basak A" first="Ajoy" last="Basak">Ajoy Basak</name><name sortKey="Cao, Xudong" sort="Cao, Xudong" uniqKey="Cao X" first="Xudong" last="Cao">Xudong Cao</name><name sortKey="Cao, Xudong" sort="Cao, Xudong" uniqKey="Cao X" first="Xudong" last="Cao">Xudong Cao</name><name sortKey="Cao, Xudong" sort="Cao, Xudong" uniqKey="Cao X" first="Xudong" last="Cao">Xudong Cao</name><name sortKey="Cao, Xudong" sort="Cao, Xudong" uniqKey="Cao X" first="Xudong" last="Cao">Xudong Cao</name><name sortKey="Huan Khieu, Nam" sort="Huan Khieu, Nam" uniqKey="Huan Khieu N" first="Nam" last="Huan Khieu">Nam Huan Khieu</name><name sortKey="Jarrell, Harold" sort="Jarrell, Harold" uniqKey="Jarrell H" first="Harold" last="Jarrell">Harold Jarrell</name><name sortKey="Majumdar, Swapan" sort="Majumdar, Swapan" uniqKey="Majumdar S" first="Swapan" last="Majumdar">Swapan Majumdar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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